Published December 2009 | Version v1
Journal article Metadata-only

Chromosome ploidy and related enzyme activities of cymbidium goeringii rhizome treated by 60Co γ-rays

Creators

  • 1. Eepartment of Horticulture, Zhejiang University, Hangzhou (China)

Description

After acute 60Co γ-rays irradiation of treated Cymbidium goeringii 'Songmei', stem tip induction, the bud induction, chromosome ploidy and cytochrome oxidase (COD), peroxidase (POD), amylase (AMY), esterase (EST) enzyme activities of rhizomes between different treatments were analyzed. Results showed that irradiation of dose above 10 Gy inhibited the bud induction and proliferation of rhizome. Low dose irradiation(≤7 Gy) induced hyperdiploid phenomenon, and all with high dose irradiation (≥10 Gy) caused hypodiploid. EST activity of Cymbidium goeringii rhizome after the treatment was higher compared to the control, POD activity and AMY activity in low dose treatment (≤ 7 Gy) were higher than that of control, while COD activity in high dose treatment (≥ 10 Gy) was higher than that of control. The results indicated that the critical dose was between in the range of 7 ∼ 10 Gy, which could be used as the optimal dose for irradiation-induced mutation of Cymbidium goeringii. (authors)

Additional details

Publishing Information

Journal Title
Acta Agriculturae Nucleatae Sinica
Journal Volume
23
Journal Issue
6
Journal Page Range
p. 971-975, 1020
ISSN
1000-8551

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
42093684
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
AMYLASE; BIOLOGICAL RADIATION EFFECTS; BUDS; CHROMOSOMES; COBALT 60; CONTROL; CYTOCHROME OXIDASE; ENZYME ACTIVITY; FLOWERS; GAMMA RADIATION; INHIBITION; LOW DOSE IRRADIATION; MUTATIONS; ORNAMENTAL PLANTS; PEROXIDASES; PLOIDY; PROLIFERATION; RADIATION DOSES; RADIOINDUCTION
Descriptors DEC
BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; COBALT ISOTOPES; DOSES; ELECTROMAGNETIC RADIATION; ENZYMES; GLYCOSYL HYDROLASES; HYDROLASES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; IRRADIATION; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; O-GLYCOSYL HYDROLASES; ORGANIC COMPOUNDS; OXIDASES; OXIDOREDUCTASES; PLANTS; PROTEINS; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; YEARS LIVING RADIOISOTOPES

Optional Information

Notes
5 figs., 1 tab., 23 refs.