PMA and human blood enhance cell transformation induced by α-particles
Creators
- 1. Institute of Radiation Medicine, Beijing (China)
- 2. GSF-Institut Fuer Strahlebiologie Munchen-Neuherberg (Germany)
Description
The purpose of this study was to observe the enhancement of alpha particles induced transformed frequency (TF) of C3H10T1/2 cells by phorbol myristate acetate (PMA) and active oxygen species released from polymorphonuclear neutrophil (PMN). 2 days and 30 min. after exposure to alpha particles of 241Am PMA or PMA stimulated human blood were added to these cell cultures. 10 days and 6 weeks later the plate efficiency (PE) and TF were determined respectively. The preliminary results obtained from 1250 dishes indicated that PMA could stimulate cell proliferation, it depended on cell densities plated and doses exposed. The enhanced trend of TF in exposed cells by PMA and PMA stimulated PMN were observed, the favourable dose was 0.5 Gy; their TF increased 2.1 and 2.8 fold by PMA and PMA stimulated PMN respectively
Additional details
Publishing Information
- Journal Title
- Journal of Radiation Research and Radiation Processing
- Journal Volume
- 14
- Journal Issue
- 4
- Journal Page Range
- p. 235-238.
- ISSN
- 1000-3436
- CODEN
- FYYXEA
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 28025148
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- ALPHA PARTICLES; BIOLOGICAL RADIATION EFFECTS; CELL CULTURES; CELL PROLIFERATION; CELL TRANSFORMATIONS; DOSE-RESPONSE RELATIONSHIPS; IN VITRO; IRRADIATION; LEUKOCYTES; RADICALS
- Descriptors DEC
- BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CHARGED PARTICLES; HELIUM IONS; IONIZING RADIATIONS; IONS; MATERIALS; ONCOGENIC TRANSFORMATIONS; RADIATION EFFECTS; RADIATIONS