Preparation and post-labelling immunoreactivity of anti-human lung cancer monoclonal antibodies

Anti-human lung cancer monoclonal antibodies (McAbs) were prepared using hybridoma technology. Two McAbs, 2E3 and 6D1, were radioiodinated with Iodogen method and the post-labelling immunoreactivity was evaluated with in vitro cell binding assay. The binding ratios of McAb 2E3 and 6D1 with lung cancer cells were 31.99% and 35.81% respectively. Two important parameters of radioiodinated McAbs, affinity constant and immunoreactive fraction, were calculated. The affinity constants of McAb 2E3 and 6D1 were 4.95 x 109L/mol and 4.23 x 109L/mol respectively and immunoreactive fractions 0.52 and 0.49 respectively. Radioimmunoimaging was performed in nude mice bearing a huamn lung cancer xenograft and 131I-McAb 2E3 and 6D1 can be localized in the xenografts. The results suggest that McAb 2E3 and 6D1 can react specifically with lung cancer cells and will be useful for guiding diagnosis and therapy of lung cancer