Biosynthesis of cerium oxide nanoparticles and their effect on lipopolysaccharide (LPS) induced sepsis mortality and associated hepatic dysfunction in male Sprague Dawley rats

Highlights: • Sepsis is the disorder of organ which causes death. • CeO₂ NPs were synthesized using eco-friendly method. • CeO₂ NPs were evaluated against lipopolysaccharide (LPS) male rats. • The Kupffer cells and macrophage cells treated with CeO₂NPs. • Results showed lowest TNF-a, IL-1b, IL-6, and HMGB1 release. - Abstract: The fetal/life challenging Sepsis is an urgent medical disaster which have developed "blood poisoning". The sepsis is one of the disorders of organs which cause death in patients. The investigational models that mimicchemical change of sepsis in human bodywereessential. We have synthesized CeO₂ NPs using eco-friendly method using plant extract. The characterization of synthesized CeO₂ NPs was studied using UV–Vis, FTIR, XRD, SEM, EDX and TEM analysis. The results were confirmed that the synthesized CeO₂ was in nano size with spherical shape. The synthesized CeO₂ nanoparticles were evaluated against lipopolysaccharide (LPS) male Sprague Dawley rats. We have intravenously administrated CeO₂NPs (0.5 mg/kg) to Sprague Dawleyratswhich are septic and checked the death rate, respiratory rate, blood pressure, body temperature. The rats could restore all parameters with decrease in hepatic damage, serum cytokines/chemokines, and swelling indicators. The Kupffer cells and macrophage cells treated with CeO₂NPs and showed lowest TNF-a, IL-1b, IL-6, and HMGB1release. Due to the ROS, iNOS, COX-2, and nuclear factor-kappa light chain, the triggered B cells (NF-kB) transcriptional action was decreased. Thus, results represents that CeO₂NPs can be applied as healing agent for liver sepsis.