Measurement of DNA damage by electrons with energies between 25 and 4000 eV

All ionizing radiations deposit energy stochastically along their tracks. The resulting distribution of energies deposited in a small target such as the DNA helix leads to a corresponding spectrum in the severity of damage produced. Most information about the probable spectra of DNA lesion complexity has come from Monte Carlo studies which model the relationship between energy deposited in DNA and damage induced. This paper aims to establish methods of determining this relationship by irradiating pBR322 plasmid DNA using low energy electrons with energies comparable with the minimum energy thought to produce critical damage. Data show that the threshold electron energy for induction of single-strand breaks is < 25 eV, and for double-strand breaks between 25 and 50 eV. (Author)