Radioimmunologic methods of prolactin determination. Comparative investigations

There exist no principle differences in the methods of radioimmunologic determination of prolactin. The essential difference is in the mode of splitting the hormone-antibody complex from the free labelled and nonlabelled prolactin. Experimental data are reported of comparative studies by use of three of the known methods of splittingbound from free prolactin: immunosorbent, double-antibody and talc adsorption method. The results of this comparative study furnish the opportunity to choose the most suitable methods for the need of clinical diagnosis. The data of the statistical processing of the results of comparative studies, plotted on standard curves and the experimental model, are shown in tables and graphs. The data on the exactness and sensitivity of the methods showed that they are able to meet the requirements of the clinical diagnostics. Most effective for Bulgarian conditions is the determination of prolactin by the talc absorption method. The latter is least time consuming, which lends convenient means to increases the number of investigations. (author)