Published January 2016 | Version v1
Journal article Metadata-only

The novel preparation of 99mTc(I)-labeled human serum albumin (HSA) nanoparticles as a SPECT imaging agent

Creators

  • 1. National Tsing Hua University, Hsinchu, Taiwan (China). Department of Biomedical Engineering and Environmental Sciences
  • 2. National Tsing Hua University, Hsinchu, Taiwan (China). Institute of Nuclear Engineering and Science
  • 3. Institute of Nuclear Energy Research, Longtan, Taiwan (China)

Description

The purpose of this study was to develop a convenient and robust method for preparation of 99mTc(I)-labeled human serum albumin nanoparticles (HSA-NPs) as a SPECT agent for tumor imaging. Efficient radiolabeling 99mTc to HSA-NPs was achieved by simply reacting [99mTc(OH2)3(CO)3]+ with the reconstituted HSA-NPs solution. The size and morphology of 99mTc(I)-HSA-NPs were analyzed and found not to significantly change after the preformed HSA-NPs were lyophilized or labeled with 99mTc(I). By incubating 99mTc(I)-HSA-NPs in rat plasma at 37 deg C for 30 h, 75 % of the radioactivity were found to be remained in the particles, suggesting good in vitro stability. (author)

Additional details

Publishing Information

Journal Title
Journal of Radioanalytical and Nuclear Chemistry
Journal Volume
307
Journal Issue
1
Journal Page Range
p. 141-150
ISSN
0236-5731
CODEN
JRNCDM

INIS

Country of Publication
Hungary
Country of Input or Organization
Hungary
INIS RN
47011152
Subject category
S60: APPLIED LIFE SCIENCES; S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
Descriptors DEI
ALBUMINS; BLOOD SERUM; IN VITRO; LABELLING; LYOPHILIZATION; NANOPARTICLES; SINGLE PHOTON EMISSION COMPUTED TOMOGRAPHY; SYNTHESIS; TECHNETIUM 99
Descriptors DEC
BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD PLASMA; BODY FLUIDS; COMPUTERIZED TOMOGRAPHY; DIAGNOSTIC TECHNIQUES; EMISSION COMPUTED TOMOGRAPHY; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PARTICLES; PROTEINS; RADIOISOTOPES; TECHNETIUM ISOTOPES; TOMOGRAPHY; YEARS LIVING RADIOISOTOPES

Optional Information

Notes
27 refs.