A thin film approach to protein crystallography

A novel approach for the investigation of proteins or macromolecules is outlined in this conceptual study. The preparation of grapho-epitaxial layers on nanotemplated substrates is proposed as an alternative to the preparation of single crystals by vapour diffusion techniques. Crystal structure investigations of such layers may then be performed via grazing-incidence diffraction (GIXRD) in the Laue mode. Quantitative expressions for the position and intensities of XRD peaks in this geometry are presented that fully consider the effects of refraction and absorption. A simulation of the Laue-GIXRD pattern of single-crystalline layers of Concanavalin A is given. The main challenges of the approach are concluded to relate to the preparation of single-crystalline protein layers. However, if those obstacles could be overcome, a 10-100-fold faster sample throughput would become possible.