Determination of lymphokine-induced cytolytic activity of macrophages by 3H-thymidine residue in prelabeled tumor cells

A method of determination of MAF-induced Mph activation by the 3H-thymidine residue in prelabeled tumor cells is described. Cells of mastocytoma P815 were used as the TC. Experiments were carried out in which MAF were revealed simultaneously by this method and by the standard method with the use of 51Cr-labeled TC. The investigation was carried out on mice. To obtain labeled TC, tumor cells were cultured with 3H-thymidine or incubated in a culture containing 51Cr. It is found that the technique for detection of lysis of 3H-thymidine-labeled tumor cells shown here possesses high sensitivity for determination of MAF-induced cytolytic activity of Mph