Ag-nanoparticle fractionation by low melting point agarose gel electrophoresis
Creators
- 1. Consejo Superior de Investigaciones Cientificas (CSIC), Instituto de Ciencia y Tecnología de Polímeros (ICTP) (Spain)
- 2. University of California, Santa Barbara, Department of Chemistry and Biochemistry (United States)
Description
The separation of surface-enhanced raman scattering (SERS)-active Ag-multi-nanoparticle (NP) assemblies by low melting point agarose gel electrophoresis was accomplished here by controlling surface charge using NP capping agents, and the pore size of agarose gel matrix. Detailed transmission electron microscopy analysis of excised gel fractions showed dimers and small clusters to have the greatest SERS activity and a mobility in between the monomers and large aggregates. This strategy enables one to: (1) stabilize small multispherical Ag clusters against further aggregation during purification; (2) fractionate and recover spherical assemblies by nuclearity; and (3) analyze SERS-enhancements for each fraction to optimize purification conditions.
Additional details
Identifiers
Publishing Information
- Journal Title
- Journal of Nanoparticle Research
- Journal Volume
- 14
- Journal Issue
- 10
- Journal Page Range
- p. 1-8
- ISSN
- 1388-0764
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 44031362
- Subject category
- S77: NANOSCIENCE AND NANOTECHNOLOGY;
- Descriptors DEI
- AGGLOMERATION; DIMERS; ELECTROPHORESIS; FRACTIONATION; GELS; MELTING POINTS; MONOMERS; NANOSTRUCTURES; PURIFICATION; RAMAN EFFECT; TRANSMISSION ELECTRON MICROSCOPY
- Descriptors DEC
- COLLOIDS; DISPERSIONS; ELECTRON MICROSCOPY; MICROSCOPY; PHYSICAL PROPERTIES; SEPARATION PROCESSES; THERMODYNAMIC PROPERTIES; TRANSITION TEMPERATURE
Optional Information
- Copyright
- Copyright (c) 2012 Springer Science+Business Media B.V.