Published March 25, 2013 | Version v1
Journal article Metadata-only

Synthesis, characterization and use of Ru-Fc intercalation complex as an electrochemical label for the detection of pathogen-DNA

Creators

  • 1. Department of Chemistry, PO Box 23346, University of Puerto Rico, Río Piedras Campus, San Juan, Puerto Rico, 00931-3346 (Puerto Rico)
  • 2. Department of Biology, PO Box CUH 100 Rd 908, University of Puerto Rico, Humacao Campus, Humacao, Puerto Rico, 00791 (Puerto Rico)

Description

This report describes the synthesis of [Ru(Fe-Phen)2dppz](PF6)2 (Ru-Fe complex) for a label-free approach to detect DNA hybridization. The Ru-Fe complex showed oxidation signals at +608 mV and +1192 mV corresponding to the RuII/III and FeII/III centers, respectively. We used the Ru-Fe complex and the Ferrocene covalently attached to the target to monitor the hybridization event of a 70-mer oligo immobilized in 10.3KD NHS-PS-NHS. The lowest target detectable concentration for the DNA fragment was around 0.4 μM.

Availability note (English)

Available from http://dx.doi.org/10.1088/1742-6596/421/1/012008

Additional details

Identifiers

DOI
10.1088/1742-6596/421/1/012008;

Publishing Information

Journal Title
Journal of Physics. Conference Series (Online)
Journal Volume
421
Journal Issue
1
Journal Page Range
[5 p.]
ISSN
1742-6596

Conference

Title
8. Ibero-American congress on sensors
Acronym
Ibersensor 2012
Dates
16-19 Oct 2012
Place
Carolina (Puerto Rico)

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
44069662
Subject category
S36: MATERIALS SCIENCE;
Resource subtype / Literary indicator
Conference
Descriptors DEI
CLATHRATES; COVALENCE; DNA; DNA HYBRIDIZATION; ECOLOGICAL CONCENTRATION; ELECTROCHEMISTRY; FERROCENE; IRON COMPLEXES; OXIDATION; PATHOGENS; RUTHENIUM COMPLEXES; SYNTHESIS
Descriptors DEC
BIOTECHNOLOGY; CHEMICAL REACTIONS; CHEMISTRY; COMPLEXES; DIENES; GENETIC ENGINEERING; HYBRIDIZATION; HYDROCARBONS; IRON COMPLEXES; NUCLEIC ACID HYBRIDIZATION; NUCLEIC ACIDS; ORGANIC COMPOUNDS; POLYENES; TRANSITION ELEMENT COMPLEXES