Published September 2008
| Version v1
Journal article
Involvement of glyceraldehyde-3-phosphate dehydrogenase in the X-ray resistance of HeLa cells
Description
We investigated changes in the sub-cellular distribution of glycelaldehyde-3-phosphate dehydrogenase (GAPDH) after X-ray irradiation in HeLa cells. Twenty-four h after irradiation at 5Gy, nuclear GAPDH levels increased 2.6-fold, whereas total GAPDH levels increased only 1.2-fold. Knockdown of GAPDH using specific small interfering RNA (siRNA) led to sensitization to X-ray-induced cell death. These results suggest that GAPDH plays a role in the radioresponse
Additional details
Publishing Information
- Journal Title
- Bioscience, biotechnology, and biochemistry
- Journal Volume
- 72
- Journal Issue
- 9
- Journal Page Range
- p. 2432-2435
- ISSN
- 0916-8451
INIS
- Country of Publication
- Japan
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 46024474
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- APOPTOSIS; HELA CELLS; IRRADIATION; OXIDOREDUCTASES; PHOSPHATES; RADIOSENSITIVITY; RNA; X RADIATION
- Descriptors DEC
- ANIMAL CELLS; ELECTROMAGNETIC RADIATION; ENZYMES; IONIZING RADIATIONS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; PROTEINS; RADIATIONS; SENSITIVITY; TUMOR CELLS
Optional Information
- Notes
- FAO/AGRIS record; ARN: JP2009000563