Breeding of a highly effective organophosphorus-degrading mutant bacteria obtained by ion beam irradiation

An organophosphorus-degrading Bacillus subtilis strain was irradiated by ¹²C⁶⁺ ion beam and a highly efficient organophosphorus-degrading LA-1 strain was selected through selective breeding medium and shake flask experiments. The effect of organophosphorous pesticide degradation was also studied. The results showed that the highly stable LA-1 mutant strain was screened through eight generation propagation at an absorbed dose of 150 Gy. The amount of degraded organophosphorus was 161.71 mg/L, which was 2.89 times higher than that of the original strain. The degradation rates were 93.5% on phoxin and 83.6% on omethoate when the concentration reached 800 μg/mL and 400 μg/mL, respectively. The LA-1 mutant strain has a promising organophosphorus pesticide degradation rate. (authors)