Published October 1982 | Version v1
Journal article

Tumor xenotransplantation in Wistar rats after treatment with cyclophosphamide and total lymphoid irradiation

  • 1. St. Radbond Academic Hospital, Nijmegen, Netherlands

Description

Three-month-old male Wistar rats were treated with cyclophosphamide and total lymphoid irradiation, and C22LR mouse osteosarcoma was transplanted into the rats. The effects of immunosuppression were monitored by lymphocyte counts, serum IgG determinations, phytohemagglutinin (PHA) and concanavalin A (Con A) responses, measurement of the proportion of B cells, and histopathological studies of the lymphoid organs. At eight days after treatment, the lymphocyte counts, IgG levels, and PHA and Con A values were decreased. Mitotic activity started in the depleted B and T cell areas of the peripheral lymphatic organs two weeks after treatment. There was a 94% graft take of the osteosarcoma. It was determined that the optimum time for tumor xenograft transplantation is 4 days after treatment. The duration of growth was 11 days, and this was followed by regression up to day 21

Additional details

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Publishing Information

Journal Title
Radiology
Journal Volume
145
Journal Issue
1
Series
Radiology.
Journal Page Range
191-194
ISSN
0033-8419