Analysis of 18F-labelled synthesis products on TLC plates: Comparison of radioactivity scanning, film autoradiography, and a phosphoimaging technique
Creators
- 1. Division of Nuclear Medicine, Helsinki University Central Hospital, P.O. Box 340, FIN-00029 Helsinki (Finland)
- 2. Turku PET Centre, Tykistokatu 6A, FIN-20520 Turku (Finland)
- 3. Laboratory of Radiochemistry, University of Helsinki, P.O. BOX 55, FIN-00014 University of Helsinki (Finland)
Description
We compared radioactivity scanning, film autoradiography, and digital photostimulated luminescence (PSL) autoradiography (phosphoimaging technique) in detection of radioactivity on thin-layer chromatography (TLC) plates. TLC combined with radioactivity detection is rapid, simple, and relatively flexible. Here, 18F-labelled synthesis products were analyzed by TLC and the radioactivity distribution on the plates determined using the three techniques. Radioactivity scanning is appropriate only with good chromatographic resolution and previously validated scanning parameters. Film autoradiography exhibits poor linearity if radioactivity varies greatly. PSL provides high sensitivity and resolution and superior linearity compared with the other methods
Additional details
Identifiers
- DOI
- 10.1016/j.apradiso.2006.04.012;
- PII
- S0969-8043(06)00202-8;
Publishing Information
- Journal Title
- Applied Radiation and Isotopes
- Journal Volume
- 64
- Journal Issue
- 9
- Journal Page Range
- p. 1043-1047
- ISSN
- 0969-8043
- CODEN
- ARISEF
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 38025422
- Subject category
- S07: ISOTOPES AND RADIATION SOURCES; S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
- Descriptors DEI
- AUTORADIOGRAPHY; COMPARATIVE EVALUATIONS; DISTRIBUTION; FILMS; FLUORINE 18; LABELLED COMPOUNDS; PHOTOLUMINESCENCE; PLATES; RADIATION DETECTION; RADIOACTIVITY; SENSITIVITY; THIN-LAYER CHROMATOGRAPHY
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; CHROMATOGRAPHY; DETECTION; EMISSION; EVALUATION; FLUORINE ISOTOPES; HOURS LIVING RADIOISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LIGHT NUCLEI; LUMINESCENCE; NANOSECONDS LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; PHOTON EMISSION; RADIOISOTOPES; SEPARATION PROCESSES
Optional Information
- Copyright
- Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.