Published April 1998
| Version v1
Journal article
Hybridization of plant virus ssRNAs Transferred to Hybond N membrane
- 1. Institute of Virology, Slovak Academy of Sciences, 84215 Bratislava (Slovakia)
- 2. Department of Microbiology and Biochemistry, Faculty of Chemical Technology, Slovak University of Tehchnology, 81237 Bratislava (Slovakia)
Description
In this paper we present a protocol for the non-denaturating agarose gel electrophoresis of plant virus ssRNAs, their blotting onto Hybond N membrane, and hybridization with [alpha 32P]dNTP-labelled cDNA probe. The protocol is not pretentious on technical equipment, omits denaturation and neutralization steps and some chemical required in other modifications. (authors)
Additional details
Identifiers
Publishing Information
- Journal Title
- Chemical Papers
- Journal Volume
- 52
- Journal Issue
- 2
- Series
- Previous name of the journal Chemical Papers was Chemicke Zvesti (used in present time, too)
- Journal Page Range
- p. 127-128
- CODEN
- CHPAEG
INIS
- Country of Publication
- Slovakia
- Country of Input or Organization
- Slovakia
- INIS RN
- 31049141
- Subject category
- S60: APPLIED LIFE SCIENCES; S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Resource subtype / Literary indicator
- Numerical Data
- Descriptors DEI
- BIOLOGICAL MARKERS; EXPERIMENTAL DATA; LABELLED COMPOUNDS; LABELLED POOL TECHNIQUES; LABELLING; MEMBRANES; METABOLISM; PHOSPHORUS 32; RNA; TRACER TECHNIQUES; VIRUSES
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DATA; DAYS LIVING RADIOISOTOPES; INFORMATION; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NUCLEI; NUCLEIC ACIDS; NUMERICAL DATA; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PARASITES; PHOSPHORUS ISOTOPES; RADIOISOTOPES; TRACER TECHNIQUES
Optional Information
- Contract/Grant/Project number
- Grants VEGA-2/3010/96; VEGA-2/4039/97
- Notes
- 1 fig., 4 refs.