Comparison the Antifungal Activity Against Fusarium Oxysporum of Some Different Essential Oil Encapsulated in Nanoemulsion

The study aims to evaluate the in vitro antifungal activity against Fusarium oxysporum of 6 plant essential oils encapsulated nanoemulsion (EO-LNs) and their combination of the 3 EOs having the highest in vitro antifungal activity. Firstly, 6 EO-LNs were prepared by a combining method of homogenization and sonication. Their binary and ternary combined formulations were also prepared by mixing with an equal volume ratio of each EO-LNs. In vitro antifungal activity of 6 EO-LNs and their combined formulations was evaluated using petri dish assay. The results indicated that 6 EO-LNs and their combined formulations were successfully prepared with the mean particle size smaller than 187 nm and the zeta potential greater than 41 mV. The results also showed that the combined formulations of EO-LNs had better antifungal activity against F. oxysporum compared to the corresponding individual EO-LNs. Especially, the combination between garlic and lemongrass essential oil (naTS) exhibited the highest in vitro antifungal effect with the growth inhibition value of 97,48% at 400 times dilution. Therefore, naTS has a great potential application as an effective biofungicide against Fusarium oxysporum. (author)