Development of radiation method for production of genetic mutant using microspore culture system. 2. Genetic effect of γ-radiation on fertilization period flower of the colza

By using cut flower culture method, effects of γ-ray radiation on mutant induction of fertilization period flower of the colza were investigated. Defining a seed formed after irradiation to M1, for M2 generation obtained by once and M3 generation obtained twice autophagy thereafter, systems different morphologically from its original kind were checked. As a result, it was confirmed that a system with rounded leaves, a system with spotted leaves, a system with fat end of pod, a system with deformed shape of pod, and so forth were found and mutant induction of radiation using cut flower culture was effective. (G.K.)