Photoelectrochemical immunosensor for HER2 detection based on BiVO₄-Bi₂S₃ heterojunction as photoactive material and CdS as signal probe

A sandwiched photoelectrochemical (PEC) sensor was developed for sensitive detection of human epidermal growth factor receptor 2 (HER2) based on BiVO₄-Bi₂S₃ heterojunction as the photoelectric material accompanied with magnetic nanoparticles for enrichment of HER2 and CdS for signal amplification. The in situ generation of Bi₂S₃ on the surface of BiVO₄ forming a BiVO₄-Bi₂S₃ heterojunction is more conducive to the transit of electron–hole pairs. Antibody-modified MNs are utilized to capture and separate HER2 from samples. After forming a sandwich immune structure, under illumination, the photocurrent shows an increasing trend with the increment of HER2 concentration. The PEC immunosensor displays a good linear concentration range between 1.00 and 1.00 × 10³ pg·mL⁻¹ and a low limit of detection down to 0.680 pg·mL⁻¹ (S/N = 3) for HER2 under a bias voltage of 0.1 V (vs. Ag/AgCl electrode). Furthermore, the sensor was successfully applied to detect HER2 in serum samples with recoveries that ranged between 96.1 and 114% with RSDs between 1.3 and 5.9%. Graphical