Published May 1991
| Version v1
Journal article
Screening for potassium channel modulators by a high through-put 86-rubidium efflux assay in a 96-well microtiter plate
- 1. American Cyanamid Co., Pearl River, NY (USA)
Description
A rapid and sensitive 86Rb efflux assay to detect chemical compounds capable of modulating the ATP-dependent potassium (KATP) channel is described. This assay, which is performed in a 96-well microtiter plate, utilizes a substrate adherent cell line as the target, requires a small amount of 86Rb as the tracer, and is a suitable system for performing the biochemical and pharmacological characterization of the KATP-channel and its activators. Because this assay is amenable to automation, it presents a useful means for high-volume screening of chemical compounds on a routine basis
Additional details
Publishing Information
- Journal Title
- Journal of Pharmacological Methods
- Journal Volume
- 25
- Journal Issue
- 3
- Series
- J. Pharmacol. Methods.
- Journal Page Range
- 185-193
- ISSN
- 0160-5402
- CODEN
- JPMED
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 22089933
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ATP; CELL CULTURES; CHEMICAL COMPOSITION; DOSE-RESPONSE RELATIONSHIPS; MAN; MEMBRANE TRANSPORT; PHARMACOLOGY; POTASSIUM; RADIOASSAY; RUBIDIUM 86; TRACER TECHNIQUES
- Descriptors DEC
- ALKALI METALS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ELEMENTS; INTERMEDIATE MASS NUCLEI; ISOMERIC TRANSITION ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; METALS; MINUTES LIVING RADIOISOTOPES; NUCLEI; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PRIMATES; RADIOISOTOPES; RUBIDIUM ISOTOPES; VERTEBRATES