Radioimmunoassay of arginine vasopressin (AVP) and clinical application
Creators
- 1. Ulm Univ. (Germany, F.R.). Sektion Nephrologie und Mineralhaushalt
- 2. Ulm Univ. (Germany, F.R.). Abt. Endokrinologie und Stoffwechsel
Description
The low circulating levels of vasopressin required an initial extraction procedure. The extraction method itself presented problems with the specificity and the reproducibility of the extracted hormone from serum. The presented paper describes the developement of a radioimmunoassay without an extraction procedure. The AVP was coupled with rabbit-albumin by gluteraldehyde condensation for the immunization of rabbits. Synthetic AVP was iodinated by the method of GREENWOOD and HUNTER (1963) and purified by the addition of Dowex. The antibody cannot differentiate between lysine- and arginine-, ornithine-, glycerine - vasopressin and oxytocin. 1 - 24 ACTH and gastrin did not crosscreact. Normal subjects were found to have 5.7 +- 4.4/uU/ml after a dehydration period of 12 hours. Subjects suffering from psychogenic polydipsia showed normal levels, however, different forms of stress yielded higher levels in normal subjects. In patients suffering from liver cirrhosis the values normalized when ascites was under control. (orig.)
Availability note (English)
MF available from INIS under the Report Number.Abstract (German)
Die niedrigen Zirkulationsspiegel des Vasopressin erforderten eine Extraktion. Das Extraktionsverfahren selbst warf Probleme auf bezueglich Spezifizitaet und Reproduzierbarkeit der aus dem Serum extrahierten Hormone. Die vorliegende Arbeit beschreibt die Entwicklung eines Radioimmunoassay ohne Extraktion. Das AVP wurde mit Kaninchenalbunin, mit Hilfe einer gluteraldehyde Kondensation zur Immunisierung von Kaninchen verbunden. Syntethisches AVP wurde unter Anwendung der Greenwood- u. Hunter-Methode (1963) iodiert und durch Zugabe von Dowex gereinigt. Der Antikoerper kann nicht zwischen Lysin-, Arginin-, Ornithin-, Glyzerin-Vasopressin und Oxidozin unterscheiden. 1-24 ACTH und Gaswin zeigten keine Kreuzreaktionen. Die Ergebnisse zeigten, dass Gesunde nach einer Dehydrationszeit von 12 Std. Werte von 5,7 +- 4,4 /uU/ml hatten. Patienten mit psychogener Polydiapsie zeigten normale Spiegel, verschiedene Formen von Stress jedoch erreichten bei normalen Patienten hoehere Spiegel. Bei Patienten mit Leberzirrhose waren die Spiegel normal, wenn der Ascites unter Kontrolle gehalten wurde. (orig.)
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Additional details
Publishing Information
- Imprint Pagination
- 7 p.
- Report number
- AED-Conf--77-461-057
Conference
- Title
- International symposium on radioimmunoassay and related procedures in medicine.
- Dates
- 31 Oct - 4 Nov 1977.
- Place
- Berlin, Germany, F.R.
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 9395265
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Resource subtype / Literary indicator
- Conference
- Descriptors DEI
- ALBUMINS; ARGININE; ASCITES; DIAGNOSTIC TECHNIQUES; IMMUNITY; IODINE 125; LIVER CIRRHOSIS; NUCLEAR MEDICINE; PATIENTS; RABBITS; RADIOIMMUNOASSAY; VASOPRESSIN
- Descriptors DEC
- AMINO ACIDS; ANIMALS; BETA DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM DISEASES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MEDICINE; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; PATHOLOGICAL CHANGES; PEPTIDE HORMONES; PITUITARY HORMONES; PROTEINS; RADIOISOTOPES; SYMPTOMS; TRACER TECHNIQUES; VERTEBRATES
Optional Information
- Notes
- 1 fig.; 9 refs.
- Secondary number(s)
- IAEA-SM--220/99.