Published March 1990 | Version v1
Journal article Metadata-only

A simple method for estimating the rate of repair synthesis in cultured mammalian cells

Creators

  • 1. AN SSSR, Leningrad (USSR). Inst. Yadernoj Fiziki

Description

A sensitive method for estimating the rate of repair synthesis is proposed. The method is based on the capacity of long-wave UV-light to inactivate cells having minor amounts of bromodeoxyuridine (BUdR) incorporated. This treatment is absolutely non-foxic for cells lacking BUdR. The efficacy of the treatment is achieved by sensitizing cells with a fluorescent dye Hoechst 33258. The proposed method has proved to be effective in studying the repair synthesis in HeLa cells after exposure to short-wave UV-light and γ-rays

Additional details

Additional titles

Original title (Russian)
Простой метод сравнительной оценки скорости репаративного синтеза в культивируемых клетках млекопитающих

Publishing Information

Journal Title
Radiobiologiya
Journal Volume
30
Journal Issue
2
Series
Radiobiologiya.
Journal Page Range
279-282
ISSN
0033-8192
CODEN
RADOA

INIS

Country of Publication
Russian Federation
Country of Input or Organization
USSR
INIS RN
22043072
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL REPAIR; BIOSYNTHESIS; BUDR; CESIUM 137; DOSE-RESPONSE RELATIONSHIPS; HELA CELLS; SURVIVAL CURVES; TIME DEPENDENCE; ULTRAVIOLET RADIATION
Descriptors DEC
ANIMAL CELLS; ANTIMETABOLITES; AZINES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; BROMOURACILS; CESIUM ISOTOPES; DRUGS; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; ISOTOPES; NUCLEI; NUCLEOSIDES; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC BROMINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RIBOSIDES; SYNTHESIS; TUMOR CELLS; URACILS; YEARS LIVING RADIOISOTOPES