Published August 2004 | Version v1
Journal article Metadata-only

The establishment of solid phase radioimmunoassay for quantitative determination of hepatitis B core antibody

Creators

  • 1. China Inst. of Atomic Energy, Beijing (China). Dept. of Isotope

Description

A sensitive and specific solid phase radioimmunoassay for hepatitis B core antibody (anti-HBc-RIA) is established. Hepatitis B core antigen (HBcAg) is coated on the solid phase beads, 125I-anti-HBc is used as the radioactive tracer. The sensitivity of the assay is 0.8 NCU/mL. The intra-assay CVs and the intre-assay CVs of 3 samples are 9.9% and 13.8% respectively. The analytical recoveries are ranged from 92.6% to 109.2%. The level of 120 normal subjects is 1.2 NCU/mL with a range of 0-2.0 NCU/mL. The method for measuring anti-HBc in human serum is rapid, sensitive, convenient and inexpensive. It is suitable for clinical and research application. (authors)

Additional details

Publishing Information

Journal Title
Journal of Isotopes
Journal Volume
17
Journal Issue
3
Journal Page Range
p. 143-146
ISSN
1000-7512

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
39002241
Subject category
S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY; S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
ANTIBODIES; CHEMICAL PREPARATION; HEPATITIS; IODINE 125; LABELLING; RADIOIMMUNOASSAY; RADIOPHARMACEUTICALS; SENSITIVITY
Descriptors DEC
BETA DECAY RADIOISOTOPES; BIOASSAY; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; DIGESTIVE SYSTEM DISEASES; DISEASES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; RADIOACTIVE MATERIALS; RADIOASSAY; RADIOIMMUNODETECTION; RADIOISOTOPES; SYNTHESIS; TRACER TECHNIQUES

Optional Information

Notes
1 fig., 10 tabs., 5 refs.