Apoptosis in immune cells induced by fission fragment 147Pm

Apoptosis in human acute lymphoblastic leukemia cell line Molt-4 cell and macrophage cell line Ana-1 cell could be induced by fission fragment 147Pm. The cumulative absorption dose of 147Pm in cultural cells through different periods were estimated. By using fluorescence microscopy and microautoradiographic tracing it can be found that Molt-4 and Ana-1 cells internally irradiated by 147Pm, displayed an obvious nuclear fragmentation and a marked pyknosis in immune cell nuclei, as well as DNA chain fragmentation and apoptotic bodies formation. The microautoradiographic study showed that 14'7Pm could infiltrate through cell membrane and displayed membrane-seeking condensation in cells. At the same time, the membrane and displayed membrane-seeking condensation in cells. At the same time, the membrane-bounded apoptotic bodies were observed. Experimental results in recent study provide evidence that Molt-4 and Ana-1 immune cells undergo apoptosis while internally irradiated with 147Pm