Published May 1, 2008 | Version v1
Journal article Metadata-only

A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry

Creators

  • 1. Insutitute for Protein Research, Osaka University, 3-2 Yamada-oka, Suita 565-0871 (Japan)
  • 2. Department of Environmental Toxicology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi, Kitakyushu 807-8555 (Japan)
  • 3. Core Research for Evolution Science and Technology, Japan Science and Technology Agency (Japan)
  • 4. Bio-multisome Research Team, Structural Physiology Research Group, RIKEN Harima Institute, Mikazuki Sayo, Hyogo 679-5148 (Japan)

Description

A vault from rat liver was crystallized in space group C2. Rotational symmetry searches indicated that the particle has 39-fold dihedral symmetry. Vault is a 12.9 MDa ribonucleoprotein particle with a barrel-like shape, two protruding caps and an invaginated waist structure that is highly conserved in a wide variety of eukaryotes. Multimerization of the major vault protein (MVP) is sufficient to assemble the entire exterior shell of the barrel-shaped vault particle. Multiple copies of two additional proteins, vault poly(ADP-ribose) polymerase (VPARP) and telomerase-associated protein 1 (TEP1), as well as a small vault RNA (vRNA), are also associated with vault. Here, the crystallization of vault particles is reported. The crystals belong to space group C2, with unit-cell parameters a = 708.0, b = 385.0, c = 602.9 Å, β = 124.8°. Rotational symmetry searches based on the R factor and correlation coefficient from noncrystallographic symmetry (NCS) averaging indicated that the particle has 39-fold dihedral symmetry

Availability note (English)

Available from http://dx.doi.org/10.1107/S0907444908004277; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2467523

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2467523;
DOI
10.1107/S0907444908004277;
PII
S0907444908004277;

Publishing Information

Journal Title
Acta Crystallographica. Section D: Biological Crystallography
Journal Volume
64
Journal Issue
Pt 5
Journal Page Range
p. 525-531
ISSN
0907-4449
CODEN
ABCRE6

INIS

Country of Publication
Denmark
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46054028
Subject category
S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
Descriptors DEI
CORRELATIONS; CRYSTALLIZATION; CRYSTALS; LIVER; PROTEINS; R FACTORS; RATS; SHAPE; SHELLS; SPACE GROUPS; SYMMETRY
Descriptors DEC
ANIMALS; BODY; DIGESTIVE SYSTEM; GLANDS; MAMMALS; ORGANIC COMPOUNDS; ORGANS; PHASE TRANSFORMATIONS; RODENTS; SYMMETRY GROUPS; VERTEBRATES

Optional Information

Copyright
Copyright (c) International Union of Crystallography 2008
Notes
PMCID: PMC2467523; PUBLISHER-ID: mh5012; PMID: 18453688; OAI: oai:pubmedcentral.nih.gov:2467523; This is an open-access article distributed under the terms described at http://journals.iucr.org/services/termsofuse.html.