Published March 21, 2008 | Version v1
Journal article Metadata-only

Crystallization and preliminary crystallographic analysis of the second GAF domain of DevS from Mycobacterium smegmatis

Creators

  • 1. School of Life Science and Biotechnology, Kyungpook National University, Daegu 702-701 (Korea, Republic of)
  • 2. Department of Biology, Yonsei University, Seoul 120-749 (Korea, Republic of)
  • 3. Department of Microbiology, Pusan National University, Pusan 609-735 (Korea, Republic of)

Description

The second GAF domain of the hypoxia-sensing DevS from M. smegmatis has been crystallized. The crystal belonged to space group P61 and diffracted to 2.0 Å resolution. Mycobacterium tuberculosis is known to transform into the nonreplicating persistence state under the influence of hypoxia or nitric oxide. DevS-DevR is a two-component regulatory system that mediates the genetic response for the transformation. DevS is a histidine kinase that contains two GAF domains for sensing hypoxia or nitric oxide. The second GAF from M. smegmatis DevS was crystallized using the sitting-drop vapour-diffusion method in the presence of sodium citrate and 2-propanol as precipitants. X-ray diffraction data were collected from crystals containing selenomethionine to a maximum resolution of 2.0 Å on a synchrotron beamline. The crystals belong to the hexagonal space group P61. The asymmetric unit contains one molecule, corresponding to a packing density of 2.5 Å3 Da−1. The selenium substructure was determined by the single anomalous dispersion method and structure refinement is in progress

Availability note (English)

Available from http://dx.doi.org/10.1107/S1744309108005186; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374252

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374252;
DOI
10.1107/S1744309108005186;
PII
S1744309108005186;

Publishing Information

Journal Title
Acta Crystallographica. Section F
Journal Volume
64
Journal Issue
Pt 4
Journal Page Range
p. 274-276
ISSN
1744-3091
CODEN
ACSFCL

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46065800
Subject category
S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
Descriptors DEI
CITRATES; CRYSTALLIZATION; CRYSTALS; DENSITY; DIFFUSION; HISTIDINE; MOLECULES; NITRIC OXIDE; PROPANOLS; SELENIUM; SODIUM COMPOUNDS; SPACE GROUPS; STOWING; X-RAY DIFFRACTION
Descriptors DEC
ALCOHOLS; ALKALI METAL COMPOUNDS; AMINO ACIDS; AZOLES; CARBOXYLIC ACID SALTS; CARBOXYLIC ACIDS; CHALCOGENIDES; COHERENT SCATTERING; DIFFRACTION; ELEMENTS; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; IMIDAZOLES; NITROGEN COMPOUNDS; NITROGEN OXIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; PHASE TRANSFORMATIONS; PHYSICAL PROPERTIES; SCATTERING; SEMIMETALS; SYMMETRY GROUPS

Optional Information

Copyright
Copyright (c) International Union of Crystallography 2008
Notes
PMCID: PMC2374252; PMID: 18391425; PUBLISHER-ID: ll5144; OAI: oai:pubmedcentral.nih.gov:2374252