Published November 1981
| Version v1
Journal article
Proliferation kinetics of perturbed cell populations determined by the bromodeoxyuridine-33258 technique: radiotoxic effects of incorporated [3H]thymidine
Description
A simple analytical method is described for the evaluation of flow cytometric data of perturbed cell populations obtained by applying the BrdUrd-33258 Hoechst technique. This procedure allows for determining the growth curve, the efflux from and the influx into G2 + M as well as the transit characteristics of cell cohorts through the G2 + M phase. As an example of the application of the method, the radiotoxic effects of incorporated 3H-thymidine on the proliferation of L-929 cells in vitro are described
Additional details
Publishing Information
- Journal Title
- Cytometry (Baltimore)
- Journal Volume
- 2
- Journal Issue
- 3
- Series
- Cytometry (Baltimore).
- Journal Page Range
- 170-174
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 13699203
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BUDR; CELL CULTURES; CELL CYCLE; CELL FLOW SYSTEMS; CELL PROLIFERATION; MITOSIS; RADIOSENSITIVITY; THYMIDINE; TOXICITY; TRITIUM COMPOUNDS
- Descriptors DEC
- ANTIMETABOLITES; ANTIMITOTIC DRUGS; AZINES; BROMOURACILS; CELL DIVISION; DRUGS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; HYDROXY COMPOUNDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC BROMINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RIBOSIDES; URACILS