Characterization and visualization of cholecystokinin receptors in rat brain using [3H]pentagastrin
- 1. National Inst. of Mental Health, Bethesda, MD
Description
[3H]Pentagastrin binds specifically to an apparent single class of CCK receptors on slide-mounted sections of rat brain (KD . 5.6 nM; Bmax . 36.6 fmol/mg protein). This specific binding is temperature-dependent and regulated by ions and nucleotides. The relative potencies of C-terminal fragments of CCK-8(SO3H), benzotript and proglumide in inhibiting specific [3H]pentagastrin binding to CCK brain receptors reinforce the concept of different brain and pancreas CCK receptors. CCK receptors were visualized by using tritium-sensitive LKB film analyzed by computerized densitometry. CCK receptors are highly concentrated in the cortex, dentate gyrus, granular and external plexiform layers of the olfactory bulb, anterior olfactory nuclei, olfactory tubercle, claustrum, accumbens nucleus, some nuclei of the amygdala, thalamus and hypothalamus
Additional details
Publishing Information
- Journal Title
- Peptides (Fayetteville, N.Y.)
- Journal Volume
- 4
- Journal Issue
- 5
- Series
- Peptides (Fayetteville, N.Y.).
- Journal Page Range
- 755-762
- ISSN
- 0196-9781
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 16034351
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BIOCHEMICAL REACTION KINETICS; BRAIN; DENSITOMETERS; GASTRIN; PHOTOMETRY; RATS; RECEPTORS; TEMPERATURE DEPENDENCE; TRACER TECHNIQUES; TRITIUM COMPOUNDS
- Descriptors DEC
- ANIMALS; BODY; CENTRAL NERVOUS SYSTEM; HORMONES; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; KINETICS; MAMMALS; MEASURING INSTRUMENTS; NERVOUS SYSTEM; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PEPTIDES; PHOTOMETERS; POLYPEPTIDES; PROTEINS; REACTION KINETICS; RODENTS; VERTEBRATES