Simultaneous assay of organic fluorine compounds in biological fluids by fluorine-19 nuclear magnetic resonance spectrometry over a large spectral width

19F-n.m.r. spectrometry is used as a direct method to assay simultaneously in body fluids (urine, plasma) all the fluorinated metabolites of a new antineoplastic drug fluoropyrimidine, 5'-deoxy-5-fluorouridine. The resonances of these metabolites are spread over a large spectral width (about 100 ppm). The calibration graphs for 5'-deoxy-5-fluorouridine and its major metabolite, α-fluoro-β-alanine, are linear over the range 10-5-10-1 M; the accuracy is 3-5% for urine samples and 5-7% for plasma samples. The method is applied to the determination of all the metabolites in urine from a patient. (Auth.)