Preparing an estrogen - derivative compound labeled with Tc-99m and determination of radiopharmaceutical potential

In recent years, estrogen derivative compounds have been used with several purpose which may lead to rapid advances in both diagnosis and therapy of some human cancers. In this study, an estrogen derivative compound (ESTCPTA) that 3,17-β-estradiolyl propinol attached with 1-(4-methylbenzoic acid)-4,8,11- tetraazacyclotetradecane (CPTA) was synthesized at five steps. Synthesized product was purified by recrystallization in ethyl alcohol. Characterizations of purified products were made by NMR and IR spectroscopy. Synthesized compound was labeled with Tc-99m. TLC (Radio Thin Layer Chromatography) and radio-paper electrophoresis were used to determine radiochemical yields. Specific activity was approximately 23.7 GBq/mmol and labeling yield was over 95%. Biodistribution studies were performed on female Albino Wistar rats. Rats were sacrificed by ether narcotization at certain time intervals and the organs were removed. Their activities were counted by a gamma counter. The activity per gram tissue was calculated and time activity curves were generated