Expression, purification and preliminary X-ray crystallographic analysis of UDP-galactopyranose mutase from Deinococcus radiodurans

UDP-galactopyranose mutase was crystallized using the microbatch method. The crystals diffracted to 2.36 Å resolution using synchrotron radiation. UDP-galactopyranose mutase (UGM) catalyzes the interconversion of UDP-galactopyranose and UDP-galactofuranose. A UGM–substrate complex from Deinococccus radiodurans has been expressed, purified and crystallized. Crystals were obtained by the microbatch-under-oil method at room temperature. The crystals diffracted to 2.36 Å resolution at the Canadian Light Source The space group was found to be P2₁2₁2₁, with unit-cell parameters a = 134.0, b = 176.6, c = 221.6 Å. The initial structure solution was determined by molecular replacement using UGM from Mycobacterium tuberculosis as a template model