Published December 2023 | Version v1
Journal article Metadata-only

Electrochemiluminescent bioassay based on Ru@Zr-BTC-MOFs nanoparticles for determination of let-7a miRNA using the hybridization chain reaction

Creators

  • 1. Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, School of Pharmacy, China Pharmaceutical University, Nanjing, People's Republic of (China)

Description

Carboxyl-rich tris(4,4′-dicarboxylic acid-2,2′-bipyridyl) ruthenium(II) ([Ru(dcbpy)3]2+) and 1,3,5-phenyl tricarboxylic acid (H3BTC) were used as the organic ligand to synthesize the metal−organic frameworks by a simple one-pot hydrothermal method with ZrCl4 as metal ion source. Subsequently, the excellent electrochemiluminescence (ECL) luminophore (denoted as Ru@Zr-BTC-MOFs) was obtained. The Ru@Zr-BTC-MOFs displayed outstanding ECL properties, and a sensitive ECL bioassay based on Ru@Zr-BTC-MOFs was designed for the detection of let-7a microRNA (miRNA) using hybrid chain reaction (HCR). Under the optimal experimental conditions, the proposed bioassay exhibited a good linear relationship in the range from 50.0 fM to 5.00 × 102 pM with a detection limit of 3.71 fM. Besides, the proposed sensor exhibited satisfactory performance in real samples. The recovery was 91 ~ 108%, and the relative standard deviation was less than 5.6%. It might have potential clinical applications for detecting miRNA in biomedical research and clinical diagnosis. Graphical

Availability note (English)

Available from http://link.springer.com/openurl/fulltext?id=doi:10.1007/s00604-023-06107-0

Additional details

Identifiers

DOI
10.1007/s00604-023-06107-0;

Publishing Information

Journal Title
Microchimica Acta (Online)
Journal Volume
191
Journal Issue
1
Journal Page Range
p. 1-8
ISSN
1436-5073

INIS

Country of Publication
Austria
Country of Input or Organization
Austria
INIS RN
56003770
Subject category
S77: NANOSCIENCE AND NANOTECHNOLOGY; S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
Descriptors DEI
BIOASSAY; BIPYRIDINES; CHEMILUMINESCENCE; DICARBOXYLIC ACIDS; GOLD; HYDROTHERMAL SYNTHESIS; NANOCHEMISTRY; NANOPARTICLES; RNA; RUTHENIUM COMPOUNDS; ZIRCONIUM CHLORIDES
Descriptors DEC
AZINES; CARBOXYLIC ACIDS; CHEMISTRY; CHLORIDES; CHLORINE COMPOUNDS; ELEMENTS; EMISSION; HALIDES; HALOGEN COMPOUNDS; HETEROCYCLIC COMPOUNDS; LUMINESCENCE; METALS; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PARTICLES; PHOTON EMISSION; PYRIDINES; REFRACTORY METAL COMPOUNDS; SYNTHESIS; TRANSITION ELEMENT COMPOUNDS; TRANSITION ELEMENTS; ZIRCONIUM COMPOUNDS; ZIRCONIUM HALIDES

Optional Information

Copyright
Copyright (c) 2023 The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature