Published July 1978 | Version v1
Journal article Metadata-only

The use of [125I]deoxyuridine for semi-microevaluation of human lymphocyte response to phytohemagglutinin in vitro

Creators

  • 1. Perugia Univ. (Italy)
  • 2. Rome Univ. (Italy)

Description

[125I]5-iodo-2'-deoxyuridine ([125I]UdR) was compared with [6-3H]thymidine ([3H]TdR) in a semi-micromethod used to evaluate the response of human lymphocytes to phytohemagglutinin (PHA) in vitro. 5-Fluoro-2'-deoxyuridine (FUdR) was added to [125I]UdR, in order to increase its incorporation, by preventing endogenous thymidine synthesis. The relationship between PHA concentration and the isotope uptake by lymphocytes was studied. Similar dose response curves were found for the two precursors, with a peak at 15 μg/ml of PHA. Nevertheless the recovery of the labeled compounds was higher when [125I]UdR together with FUdR was used. (Auth.)

Additional details

Identifiers

DOI
10.1016/0022-1759(78)90071-6;

Publishing Information

Journal Title
Journal of Immunological Methods
Journal Volume
22
Journal Issue
1-2
Series
J. Immunol. Methods.
Journal Page Range
191-196
ISSN
0022-1759

INIS

Country of Publication
Netherlands
Country of Input or Organization
Netherlands
INIS RN
9417709
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BLOOD; IMMUNOLOGY; IN VITRO; IODINE 125; IODODEOXYURIDINE; LABELLED COMPOUNDS; LYMPHOCYTES; PHYTOHEMAGGLUTININ; QUANTITY RATIO; THYMIDINE; TRITIUM
Descriptors DEC
AGGLUTININS; ANIMAL CELLS; ANTIMETABOLITES; ANTIMITOTIC DRUGS; AZINES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD CELLS; BODY FLUIDS; CARBOHYDRATES; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; HEMAGGLUTININS; HETEROCYCLIC COMPOUNDS; HYDROGEN ISOTOPES; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; IODOURACILS; ISOTOPES; LEUKOCYTES; LIGHT NUCLEI; MUCOPROTEINS; NUCLEI; NUCLEOSIDES; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC IODINE COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; POLYSACCHARIDES; PROTEINS; PYRIMIDINES; RADIOISOTOPES; RIBOSIDES; SACCHARIDES; SOMATIC CELLS; URACILS; YEARS LIVING RADIOISOTOPES

Optional Information

Notes
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