Published April 1984
| Version v1
Journal article
Preparation and one-step purification of mono-125I-angiotensin II for radioligand binding assays
Description
A one-step purification of mono-125I-angiotensin II prepared by the chloramine T procedure is described. The purification is effected on a cellulose cation exchange column with isocratic elution by 50 mM sodium acetate, pH 5.0. The purity of the mono-125I-angiotensin II was determined by thin layer chromatography, high pressure liquid chromatography, enzymatic digestion, radioreceptor assay, and radioimmunoassay. Preparation and purification of mono-125I-angiotensin II by this procedure offers significant advantages over existing methods for its preparation in terms of purity, simplicity, efficiency, and cost
Additional details
Publishing Information
- Journal Title
- J. Pharmacol. Methods
- Journal Volume
- 11
- Journal Issue
- 2
- Series
- J. Pharmacol. Methods.
- Journal Page Range
- 137-150
- ISSN
- 0160-5402
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 16005217
- Subject category
- S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ANGIOTENSIN; IODINE 125; ION EXCHANGE CHROMATOGRAPHY; LABELLED COMPOUNDS; LABELLING; LIQUID COLUMN CHROMATOGRAPHY; PURIFICATION; RADIOCHEMISTRY; RADIOIMMUNOASSAY; RATS; THIN-LAYER CHROMATOGRAPHY
- Descriptors DEC
- ANIMALS; BETA DECAY RADIOISOTOPES; CARDIOVASCULAR AGENTS; CHEMISTRY; CHROMATOGRAPHY; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; GLOBULINS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PROTEINS; RADIOISOTOPES; RODENTS; SEPARATION PROCESSES; TRACER TECHNIQUES; VASOCONSTRICTORS; VERTEBRATES