Effects of triptolide on adherence of neutrophil and rat cardiac microvascular endothelial cells

Objective: To study effects of Triptolide on adherence of neutrophil and rat cardiac microvascular endothelial cells (RCMECs). Methods: RCMECs were cultured and hypoxia-reoxgenation model of endothelial cells were developed. Neutrophil were isolated from blood, adhesion rate of endothelial cells and neutrophil were determined by the adhesion model. NF-κB activity of endothelial cells was determined by electrophoretic mobility shift assays (EMSA). Results: NF-κB activity, adherence rate of neutrophil and RCMECs treated with hypoxia were increased markedly, and after reoxgenation, these changes were more obvious. When endothelial cells were treated by reoxgenation supplemented with Triptolide, adhesion rate of endothelial cells was decreased markedly in a dose-dependent manner; NF-κB activity was decreased apparently compared with H/R (P<0.01). Conclusion: Triptolide inhibits adhesion rate of RCMECs and neutrophil mediated apparently by NF-κB. (author)