On rationalisation in the RIA Lab

With the aim of rationalisation in the RIA Lab various solid phase RIA kits for T4 were compared to a liquid phase RIA kit. The T4 solid phase RIA kits demonstrated comparable quality in regard to precision and stability towards prolongued incubation. Furthermore, a batch-wise operating partly mechanised pipetting machine (RIA Sample Processor) was submitted to clinical-laboratorial testing. Following changes in computer programme and mechanic precision was comparable to that of manual pipetting. The use of solid phase radioassay employing antibodies bound to glass particles as well as the tested RIA Sample Processor are important measures of rationalisation from approximately 50 samples in duplicates on. One technician is able to set up larger series or different radioassays one after each other. Coefficients of variation of partly mechanised pipetted radioassays were almost exclusively lower than 10% (T4/FT4-RIA, T3-RIA, TBG-Radioassay). (orig.)