Aging and phytoavailability of newly introduced and legacy cadmium in paddy soil and their bioaccessibility in rice grain distinguished by enriched isotope tracing

Highlights: • Newly introduced and legacy Cd (Cd_N/Cd_L) were distinguished by using ¹¹²Cd tracer. • Cd_N aged quickly and its isotope exchangeable pool was the same with Cd_L after aging. • Grain Cd contents were linearly related to Cd concentrations in overlying water. • Slaked lime and acidic biochar decreased and enhanced Cd phytoavalability, respectively. Phytoavailability of Cadmium (Cd) plays a critical role in its accumulation in soil-rice systems. However, differential aging and phytoavailability of newly introduced Cd (Cd_N) and legacy Cd (Cd_L) in the soil-rice system remains unknown. Moreover, distinguishing their aging and phytoavailability is challenging. Enriched ¹¹²Cd isotope was introduced into a series of pot experiments, combined with sequential extraction and isotope dilution (¹¹⁰Cd isotopic spike), to investigate the aging and distribution of Cd_N and Cd_L under different treatments. The treatments included simulated acid rain, slaked lime, and biochar. Cd_N aged quickly than Cd_L in flooded soil and its availability was similar to that of Cd_L after tillering stage. The grain Cd contents were positively correlated to Cd concentrations in the overlying water. Acid rain reduced the soil pH, increasing the grain Cd, while slaked lime reduced grain Cd content. The acidic biochar used in this study increased grain Cd, possibly through soil acidification-induced Cd release. The differences in bioaccumulation and translocation factors between Cd_N and Cd_L in rice plants under slaked lime and biochar treatments suggested their different in vivo complexations and translocations. Analysis of bioaccessibility of Cd_N and Cd_L in rice grains provided valuable insights regarding human Cd exposure.