Synthesis of D-Xylose-U-13C5

D-Xylose-U-13C5 was synthesized with D-glucose-U-13C6 as starting material via process of protection with double acetone, selective hydrolysis with acid, oxidation, reduction and hydrolysis with base. The total yield of D-Xylose-U-13C5 was 38.6% base on D- glucose-U-13C6. The target compound and their intermediates were analyzed and characterized by GC, GC-MS, HPLC and LC-MS, accordingly. The results showed that their isotopic abundance and purity could meet the required specification. The structure of final product was D-Xylose-U-13C5, 13C abundance was 99.1%, and chemical purity was 99.9%, which were high enough for use in 13C breath test. (authors)