Cellular radiosensitization by wortmannin

Objective: To examine the radiosensitization possibility of wortmannin (WT0 on some cell lines. Methods: Cells were prepared with WT or DMSO (solvent) for 1 h, and irradiated at the indicated dose. at different times after irradiation, the cells were washed to remove the WT and allowed to grow for 7 days, and colonies of more than 50 cells were examined under low power microscope. Results: Marked radiosensitizing effect of WT in concentration over 50 μmol/L on LP cells within 6 h after radiation exposure was displayed, the D0 radiosensitization ratio in the dose survival curve being around 2. WT in concentration 50 μmol/L had definite radiosensitizing effect on HeLa and SP/20 cells. Conclusion: WT is a novel-dio-sensitizer