Diagnostic transmission electron microscopy

Background. Transmission electron microscopy (TEM) is an important diagnostic tool in surgical pathology. Material and methods. We give an overview based on our own experience of the application of TEM in diagnostic pathology. Results. In spite of its potential for high-grade resolution, the actual use of TEM; in diagnostic pathology is rather limited. TEM is mandatory in the examination of kidney and muscle biopsies and is often indicated when a metabolic disease is suspected. However, in many centres its importance in tumour pathology has declined because of the advances in the field of immunohistochemistry. TEM requires a great deal of resources and high levels of skills in tissue processing and diagnostic interpretation. Interpretation. The TEM diagnosis must be integrated with high microscopical and often immuno histopathological findings as well as with the clinical data. We therefore recommend close collaboration between the clinician and the laboratory with regard to biopsy, indication, handling of samples and the final diagnosis. 3 figs., 26 refs