Cytokines production by different populations of macrophages following radiation of combined radiation injury

The study on production of the IL-1β, IL-6 and TNFα polyfunctional cytokines by peritoneal, splenic and bone-marrow macrophages after the whole-body irradiation and combined radiation-thermal injury (CRTI) is carried out. The mice-males were subjected to the single-time irradiation in the dose of 7 Gy at the LUCH γ-facility with the 60Co source. The dose rate constituted 0.45 Gy/min. By modeling the CRTI the deep thermal burn of 10% of the whole body surface square was inflicted by means of a strong light flare, following the irradiation. The cytokines production was studied in various periods of time after the irradiation and CRTI. It is established, that macrophages, harvested from the main mice hematopoietic organs do not manifest any indications of increasing the cytokines endogenic production during the first three days after the irradiation and CRTI. The increase in the level of the IL-6 and IL-1 stimulated production proved to be characteristic only of te peritoneal macrophages. No reliable differences in the level of the cytokine-producing activity of the macrophages, harvested from the mice, subjected only to the irradiation and from the mice with the combined radiation injury were revealed