Establishment of the MDR model in rabbit liver with VX2

Objective: To investigate the feasibility of establishing tumor model of MDR in rabbit liver with VX2 tumor induced by adriamycin. Methods: 20 white rabbits were randomly divided into 4 groups with 5 for each. Liver tumor was established with VX2 tissue together with or without MDR-induced for their formations. VX2 tumor without MDR-induced was included in group A and B, while MDR-induced tumor was employed in groupe C and D. Contrast enhanced CT was performed three weeks later to assess the volume of tumors. 4 mg adriamycin (dilute with 2 ml of saline) was infused in group A and C via the common herpatic artery directly and respectirely; whereas same amount of saline adminisfrated through the same way for group B and D. Follow up CT was performed one week later to assess the change of tumor. Apoptosis rate was analized for each group by means of flow cytometry (FCM). Results: No significant difference was found between the 4 groups three weeks after the establishment in term of tumor volume. Follow up CT performed 1 week after the treatment showed the volume increase of tumor for all groups. Group A was significantly lower than the other three groups in tumor growth rate (P<0.05). No significant difference was found between the other three groups (P>0.05). The apoptosis rate of group A detected by FCM was significantly higher than the other three groups (P<0.05). No significant difference was found between group B, C and D (P>0.05). Conclusion: Implanted liver tumor with VX2 induced by , adriamycin in tumor carrying rabbits possesses the character of drug resistance, which is profitable to be a liver tumor model of MDR for the therapeutic trial. (authors)