Published March 1984 | Version v1
Journal article Metadata-only

Properties of antibodies to thymine glycol, a product of the radiolysis of DNA

Creators

  • 1. New York Medical Coll., Valhalla

Description

Anti-thymine glycol antibodies were elicited by immunizing rabbits with thymine glycol monophosphate (TMP-glycol) conjugated by carbodiimide to BSA. The antibodies produced are specific for thymine glycol as measured by immunoprecipitation of TMP-glycol-RSA conjugates and hapten inhibition of reactivity with OsO4-treated DNA in an enzyme immunoassay. Using the enzyme immunoassay, the antibody is capable of detecting femtomole and picomole levels of thymine glycol in direct and competitve assays, respectively. This immunochemical assay is potentially suitable for measuring the production and repair of thymine glycol damage in cellular DNA

Additional details

Publishing Information

Journal Title
Radiat. Res.
Journal Volume
97
Journal Issue
3
Series
Radiat. Res.
Journal Page Range
499-510
ISSN
0033-7587

INIS

Country of Publication
United States
Country of Input or Organization
United States
INIS RN
15063609
Subject category
S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
Descriptors DEI
ANTIBODIES; BIOASSAY; CARBON 13; DNA; GLYCOLS; NUCLEAR MAGNETIC RESONANCE; RADIOLYSIS; THYMIDYLIC ACID; X RADIATION
Descriptors DEC
ALCOHOLS; CARBON ISOTOPES; CHEMICAL RADIATION EFFECTS; CHEMICAL REACTIONS; DECOMPOSITION; ELECTROMAGNETIC RADIATION; EVEN-ODD NUCLEI; HYDROXY COMPOUNDS; IONIZING RADIATIONS; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; NUCLEI; NUCLEIC ACIDS; NUCLEOTIDES; ORGANIC COMPOUNDS; PERFORMANCE TESTING; RADIATION EFFECTS; RADIATIONS; RESONANCE; STABLE ISOTOPES; TESTING