The research of radiation injuries induced by C-arm X ray scan

Objective: To investigate radiation injuries induced by C-arm X ray scan in mice. Methods: 72 healthy male kunming mice were randomly divided into control group, single, twice, four, eight and sixteen times of C-arm X ray scan. The mice were administered with different frequency of C-arm X ray scan; After 24 h, T-AOC, GSH and ROS were detected by T-AOC Assay Kit (ABTS method), GSH Assay Kit and ROS Assay Kit, respectively. The γ-H2AX level in peripheral lymphocytes and bone marrow cells (cell cycle phase) were analyzed by flow cytometry. Results: Compared with control, four, eight and sixteen times of scan increased ROS level, but eight and sixteen times of scan decreased GSH-level, sixteen times of scan significantly decreased T-AOC levelin thyroid. Compared with control, four, eight and sixteen times of scan increased γ-H2AX level in peripheral lymphocytes, eight and sixteen times of scan increased γ-H2AX level in bone marrow cells. Compared with control, sixteen times of scan increased the percentages of G₀/G₁, and G₂/M phases, while eight and sixteen times of scan decreased the percentages of S phases. Conclusion: C-arm X ray scan produced different degrees of radiation injuries in mice. (authors)