Published July 30, 2005
| Version v1
Journal article
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of LysA (Rv1293) from Mycobacterium tuberculosis
- 1. EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D-22603 Hamburg (Germany)
- 2. UCLA-DOE Laboratory of Structural Biology and Molecular Medicine, 206 Boyer Hall, Box 951570, Los Angeles, CA 90095-1570 (United States)
Description
M. tuberculosis diaminopimelate decarboxylase, the enzyme that catalyzes the final step of lysine biosynthesis, has been cloned, expressed, purified and crystallized in the absence of cofactor or substrate. Diaminopimelate decarboxylase from Mycobacterium tuberculosis (LysA, DAPDC, Rv1293) has been cloned and heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized. Preliminary diffraction data analysis suggests the presence of a homotetramer in the asymmetric unit
Availability note (English)
Available from http://dx.doi.org/10.1107/S1744309105022839; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1952341Additional details
Identifiers
- URL
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1952341;
- DOI
- 10.1107/S1744309105022839;
- PII
- S1744309105022839;
Publishing Information
- Journal Title
- Acta Crystallographica. Section F
- Journal Volume
- 61
- Journal Issue
- Pt 8
- Journal Page Range
- p. 782-784
- ISSN
- 1744-3091
- CODEN
- ACSFCL
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 46061209
- Subject category
- S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
- Descriptors DEI
- CRYSTALLIZATION; DATA ANALYSIS; ESCHERICHIA COLI; LYSINE; SUBSTRATES; X-RAY DIFFRACTION
- Descriptors DEC
- AMINO ACIDS; BACTERIA; CARBOXYLIC ACIDS; COHERENT SCATTERING; DATA PROCESSING; DIFFRACTION; MICROORGANISMS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PHASE TRANSFORMATIONS; PROCESSING; SCATTERING
Optional Information
- Copyright
- Copyright (c) International Union of Crystallography 2005
- Notes
- PMCID: PMC1952341; PMID: 16511157; PUBLISHER-ID: vr5048; OAI: oai:pubmedcentral.nih.gov:1952341