Published September 1982
| Version v1
Journal article
Isolation of Chinese hamster ovary cells with reduced unscheduled DNA synthesis after UV irradiation
Creators
- 1. Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands
Description
A simple procedure has been worked out to obtain UV-sensitive mutants of Chinese hamster ovary (CHO) cells. In this procedure, conventional mutagenesis is followed by BrdU--light treatment to enrich the population for UV-sensitive cells. Colonies that are allowed to form subsequently are duplicated by replica plating and screened on the master plate for their UV sensitivity and their capacity to carry out UV-induced DNA repair synthesis. Putative mutants are isolated from the replica. With this combination of methods, we succeeded in isolating CHO mutants with an 85-95% reduced level of UV-induced DNA synthesis in combination with an increased UV sensitivity
Additional details
Publishing Information
- Journal Title
- Somatic Cell Genet.
- Journal Volume
- 8
- Journal Issue
- 5
- Series
- Somatic Cell Genet.
- Journal Page Range
- 635-642
- ISSN
- 0098-0366
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 14754651
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL REPAIR; BIOSYNTHESIS; CELL CULTURES; DNA; HAMSTERS; MUTAGENESIS; MUTANTS; OVARIES; ULTRAVIOLET RADIATION
- Descriptors DEC
- ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; BODY; ELECTROMAGNETIC RADIATION; FEMALE GENITALS; GONADS; MAMMALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; RADIATION EFFECTS; RADIATIONS; RODENTS; SYNTHESIS; VERTEBRATES