Effects of γ irradiation on bone cells in vitro
Creators
- 1. Fudan Univ., Shanghai (China). Inst. of Radiation Medicine, Dept. of Bone Metabolism
Description
To study the influence of radiation on osteoblasts and osteoclasts in vitro. 137Cs γ rays was used to irradiate the rat calvaria-derived osteoblasts and femur-derived osteoclasts. The changes in morphology, proliferation rate, ALP activity, mineralized nodules forming capacity and osteocalcin mRNA levels of irradiated-osteoblasts and osteoclastic apoptosis rate were detected respectively. Degenerative changes in morphology was observed and the inhibitory effects on osteoblasts proliferation rate by radiation was correlated with dose and ALP activity was also decreased by 28%. The nodules numbers was increased by 91.75% in lower-dose (0.5-1 Gy) and decreased by 67.13% in higher-dose (6 Gy). Osteocalcin transcription levels were 1.46 in 6 Gy and 2.75 in control. The osteoclastic apoptosis rate was increased with irradiation dose. Low-dosage γ irradiation may accelerate the aging process of osteoblasts and induce osteoclasts apoptosis in vitro
Additional details
Publishing Information
- Journal Title
- Journal of Radiation Research and Radiation Processing
- Journal Volume
- 20
- Journal Issue
- 1
- Journal Page Range
- p. 40-46
- ISSN
- 1000-3436
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 34022679
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BIOLOGICAL FUNCTIONS; BONE CELLS; CELL PROLIFERATION; CESIUM 137; DOSE-RESPONSE RELATIONSHIPS; GAMMA RADIATION; IN VITRO; MORPHOLOGICAL CHANGES; MORTALITY; OSTEOPOROSIS; RADIATION DOSES; SCANNING ELECTRON MICROSCOPY; TISSUE CULTURES
- Descriptors DEC
- ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CESIUM ISOTOPES; CONNECTIVE TISSUE CELLS; DISEASES; DOSES; ELECTROMAGNETIC RADIATION; ELECTRON MICROSCOPY; INTERMEDIATE MASS NUCLEI; IONIZING RADIATIONS; ISOTOPES; MICROSCOPY; NUCLEI; ODD-EVEN NUCLEI; RADIATIONS; RADIOISOTOPES; SKELETAL DISEASES; SOMATIC CELLS; YEARS LIVING RADIOISOTOPES