Reduced graphene oxide based nanobiocomposite as basis for flexible biosensors

Flexible selective electrochemical biosensor based on reduced graphene oxide film and short oligonucleotides (aptamers) was developed. Laser scribing was applied for graphene reduction due to controllable reduction rate and simple devises pattering. Optimal parameters of film reduction were determined for effective aptamers coupling, by varying laser output power. Mild reduced graphene oxide, as was expected, revealed better reactivity for aptamers coupling. Resistive response to biosensors exposure to thrombin and albumin proteins was measured. As a result we developed bionanocomposite that can be used in a new generation of available low-cost biosensors. (paper)