Published February 27, 2013 | Version v1
Journal article Metadata-only

Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB8

Creators

  • 1. Niigata University, 8050 Ikarashi 2-no-cho, Nishi-ku, Niigata 950-2181 (Japan)
  • 2. The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562 (Japan)
  • 3. RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045 (Japan)

Description

Peptidyl-tRNA hydrolase from T. thermophilus has been expressed, purified and crystallized. The crystals diffracted X-rays to atomic resolution (beyond 1.0 Å resolution). Peptidyl-tRNA is produced from the ribosome as a result of aborted translation. Peptidyl-tRNA hydrolase cleaves the ester bond between the peptide and the tRNA of peptidyl-tRNA molecules, to recycle tRNA for further rounds of protein synthesis. In this study, peptidyl-tRNA hydrolase from Thermus thermophilus HB8 (TthPth) was crystallized using 2-methyl-2,4-pentanediol as a precipitant. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 47.45, b = 53.92, c = 58.67 Å, and diffracted X-rays to atomic resolution (beyond 1.0 Å resolution). The asymmetric unit is expected to contain one TthPth molecule, with a solvent content of 27.13% (VM = 1.69 Å3 Da−1). The structure is being solved by molecular replacement

Availability note (English)

Available from http://dx.doi.org/10.1107/S1744309113003424; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606586

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606586;
DOI
10.1107/S1744309113003424;
PII
S1744309113003424;

Publishing Information

Journal Title
Acta Crystallographica. Section F
Journal Volume
69
Journal Issue
Pt 3
Journal Page Range
p. 332-335
ISSN
1744-3091
CODEN
ACSFCL

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46082047
Subject category
S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
Descriptors DEI
CRYSTALLIZATION; CRYSTALS; MOLECULES; RESOLUTION; SOLVENTS; SPACE GROUPS; SYNTHESIS
Descriptors DEC
PHASE TRANSFORMATIONS; SYMMETRY GROUPS

Optional Information

Copyright
Copyright (c) International Union of Crystallography 2013
Notes
PMCID: PMC3606586; PMID: 23519816; PUBLISHER-ID: hc5153; OAI: oai:pubmedcentral.nih.gov:3606586