Influence of the volume ratio of the 99mTc-HSA complex to dialysis fluid on the stability of the complex during incubation (short communication)

Dynamic development of peritoneal dialysis (PD) and necessity of systematic monitoring of peritoneal kinetic parameters in long-term CAPD patients are the reason for investigation of new and save, intraperitoneal volume markers. One of these markers can be 99mTc-HSA. The necessary condition of its applicability for kinetic studies in PD is the stability of the complex in dialysis fluid during the 4-hrs CAPD exchange. The aim of the present investigation was the evaluation of the 99mTc-HSA stability in standard dialysis fluid in different dilutions. The stability was assessed using paper and column radiochromatography. Our results indicate that incubation of the complex for up to 3 hrs in the dialysis fluid at 1:1 ratio did not affect its stability (1.54% of the free 99mTcO4- ion) while similar incubation at 1:10 ratio led to a decay of the complex (20% of the free 99mTcO4- ion). It is concluded that the 99mTc-HSA complex can be a useful marker for the assessment of intraperitoneal volume of dialysing fluid but its application for examination of the transport parameters during peritoneal dialysis is controversial. (author)